Attention: you are currently viewing the conference website for the previous conference on April 22, 2022. For information on the next conference on September 8-9, 2022, please switch to the current conference webpage. Toggle website
Dr. Natalia Trufanova

Dr. Natalia Trufanova

Biology

Postdoc

Institute for Problems of Cryobiology and Cryomedicine of National Academy of Science of Ukraine

CV: Natalia A. Trufanova (29.04.1982) Senior Researcher in Cryobiochemistry Department, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, 23, Pereyaslavskaya str., Kharkiv, Ukraine PhD degree in Cryobiology Member of UNESCO Chair in Cryobiology Assistant professor in Karazin’s Kharkiv national university, Kharkiv, Ukraine Skills: 2D and 3D Cell culture, alginate microspheres production, low-temperature cell preservation (conventional freezing, vitrification), cell storage at ambient temperature, cell viability tests, bio-medical analytics

Presenting about:

3D MESENCHYMAL STEM CELL BASED CONSTRUCTS (SPHEROIDS, ALGINATE MICROSPHERES, MACROPOROUS STRUCTURES) ENGINEERING AND PRESERVATION FOR BIOMEDICAL PURPOSES

Abstract: 3D MESENCHYMAL STEM CELL BASED CONSTRUCTS (SPHEROIDS, ALGINATE MICROSPHERES, MACROPOROUS STRUCTURES) ENGINEERING AND PRESERVATION FOR BIOMEDICAL PURPOSES Natalia Trufanova, Olena Rohulska, Oleksandra Shalina, Svetlana Mazur, Olena Revenko, Olha Tykhvynska, Alexander Petrenko Institute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine, Kharkiv, Ukraine Mesenchymal stromal cells (MSCs) possess unique biological potential. Culture in different constructs (spheroids, alginate microspheres, scaffolds) affects MSCs state. The efficient preservation strategy is essential for such constructs availability. Cryopreservation is the only approach for long-term storage. However, it may result in decreased cell viability and disturbed construct structure. Preservation at ambient temperatures is an alternative in case of short-term storage. Each construct type needs customized preservation protocols which are not available up to date. We studied MSCs properties during 3D culture, non-freezing storage, and cryopreservation. Obtained results may become a basis for the engineering and banking ready-to-use MSCs-based structures with programmed properties.